CsMIXTA may play a crucial role in the morphology and development of glandular trichomes in cannabis female flow-ers.To investigate its regulatory mechanism,a 2199 bp promoter sequence of CsMIXTA was cloned.PlantCARE prediction iden-tified multiple hormone response elements and stress response elements within this region.Based on this analysis,five 5'end deletion fragments of the promoter with varying lengths were amplified.Six GUS gene expression vectors were constructed using the full-length promoter and the 5'end deletion fragments,which were then transiently expressed in tobacco leaves and industrial hemp sugar leaves.GUS staining revealed that nucleotide positions-393 and-99 constituted the core region of the CsMIXTA promoter,containing the gibberellin response element TATC-box and the transcription initiation element TATA-box.The results also demonstrated that CsMIXTA is specifically expressed in glandular trichomes of industrial hemp.Promoter activity of the core region was confirmed by luciferase assay.Stress response analysis indicated that low temperature,abscisic acid(ABA),and gib-berellin(GA3)enhanced promoter activity.These findings provide a crucial basis for further studies on the regulation of CsMIXTA.
关键词
工业大麻/CsMIXTA启动子/瞬时表达/GA3、ABA处理
Key words
industrial hemp/CsMIXTA promoter/transient expression/GA3 and AB A treatment
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