Faba bean(Vicia faba L.)is an important crop known for its high protein content,which includes all eight essential amino acids in a balanced manner.Mining protein-related genes in faba bean not only contributes to the breeding of high-quality protein varieties but also holds great significance for meeting the future demand of plant protein.In this study,a total of 320 faba bean germplasms were evaluated for protein content across three environments(Xining,Huzhu,and Huangyuan in Qinghai pro-vince)over one year.Additionally,132 selected SSR makers were used to conduct association analysis.The results showed that the protein content ranged from 19.51％to 54.34％.The coefficients of variation for the three environments were 10.835,20.865,and 13.380,respectively,displaying a normal distribution with phenotypic diversity.Among the 320 materials,778 polymorphic loci were detected by the 132 markers.The average allele number was 6,with a range of 3 to 12.The polymorphism information content(PIC)varied from 0.1527(V1797)to 0.8225(SSR-12192),with a mean value of 0.5583.Markers with a PIC value greater than the mean accounted for 49.24％of the total markers,indicating relatively high genetic diversity among the selected markers.Genetic structure analysis showed that the 320 materials could be divided into two subgroups.Subgroup Ⅰ consisted of 176 mate-rials,subgroup Ⅱ contained 107 materials,while the remaining 37 materials did not exhibit distinct group affiliation,representing a mixed taxon.Overall,the genetic diversity of the marker genes was high,and the test population displayed relatively homoge-neous structure.Through GLM and MLM analyses,a total of 22 SSR markers significantly correlated with protein content were identified.Notably,three SSR markers(SSR-10894,SSR-12695,V1929)displayed a strong association with protein content in this study.Among them,SSR-13584 exhibited consistent correlation across multiple environments,with an explanatory rate rang-ing from 4.07％to 5.19％.V1929 showed a high correlation with protein content in Huangyuan county,with explanatory rate of 10.00％and 9.20％using both correlation analysis models.The findings of this study provide a theoretical basis for parental selec-tion,faba bean protein-related gene mining,and quality breeding.
维普
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