Rice(Oryza sativa L.)is one of the world's major food crops.As the largest rice producer globally,China faces sig-nificant challenges annually due to rice blast caused by Magnaporthe oryzae.This disease can affect rice throughout its entire growth period,leading to substantial yield reductions and even total crop failure.Notably,alternative oxidase(AOX),a terminal oxidase in the mitochondrial respiratory electron transport chain,is widely present in both plants and fungi.AOX is known to play a role in regulating plant growth and development under stress conditions and in the fungal stress response.In this study,we con-ducted a comparative analysis of the structural differences between rice AOX and M.oryzae AOX proteins and employed AOX inhibitors to assess their potential in inhibiting and controlling M.oryzae.Our results revealed that while the amino acid residues in the dual-iron catalytic active center of rice and M.oryzae AOX proteins are relatively conserved,there are significant differ-ences in the size of the ubiquinone channel,amino acid composition,and the shape of the hydrophobic pocket.Treatment with AOX inhibitors significantly slowed the growth of M.oryzae hyphae and reduced the severity of disease symptoms on rice leaves.These findings suggest that AOX is a promising target for inhibiting M.oryzae,offering potential application value in the control of rice blast disease.
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