Phylogenetic and functional analysis of the BnaSLY1 genes in Brassica napus L.
Gibberellins regulate plant epidermal cell growth,stem and leaf expansion,and plant architecture.In Arabidopsis,SLY1 encodes an F-box protein that modulates plant growth by targeting the negative regulator of GA signaling,the DELLA pro-tein,for ubiquitination and subsequent degradation.However,the function of BnaSLY1 in Brassica napus has not been previously revealed.In this study,we characterized the expression patterns and performed a phylogenetic analysis of BnaSLY1.Using CRISPR/Cas9 technology,we generated mutants with different copy numbers of BnaSLY1.By integrating RNA-Seq analysis,we investigated the biological functions of BnaSLY1 and its impact on the growth and development of Brassica napus.Our results showed that there are two copies of SLY1 in Brassica napus,with similar expression patterns and constitutive expression.The protein is localized in the nucleus and is highly conserved among different varieties of rapeseed and cruciferous plants.Pheno-typic analysis of mutants revealed that,compared to the control,single mutants bnaa01sly1 and bnaa06sly1 exhibited delayed flowering and significantly reduced plant height,while the double mutant bnasly1 showed a dark green leaf phenotype,increased leaf thickness,further delayed flowering,and further reduced plant height.RNA-Seq analysis between Westar and bnasly1 showed significant enrichment of differentially expressed genes in the auxin signaling pathway and wax biosynthesis pathway,with several flowering time-related genes showing significant expression changes.This study demonstrates that BnaSLY1 not only influences plant height and flowering time but also affects epidermal wax synthesis,thereby laying a theoretical foundation for exploring the crucial role of the GA signaling pathway in the growth and development of Brasscia napus.
万方数据
维普
