Preliminary investigation of the SiLTP1:a lipid transfer protein gene involved in the salt tolerance of foxtail millet
Soil salinization poses a significant barrier to agricultural productivity.Enhancing the salt tolerance of crops is crucial for the effective utilization of saline-alkali land,thereby ensuring food security and improving agricultural efficiency.Lipid trans-fer proteins (LTPs) are a class of small,widely distributed proteins in higher plants,playing crucial roles in plant growth,signal transduction,and responses to various biotic and abiotic stresses.In a previous study,a lipid transfer protein gene,designated SiLTP1,was cloned from foxtail millet.In this study,we constructed both a prokaryotic expression vector and a plant binary overexpression vector for SiLTP1.The prokaryotically expressed protein and four homozygous transgenic lines were successfully obtained and characterized.In vitro salt tolerance assays revealed that the SiLTP1 protein exhibited a notable degree of salt toler-ance.Physiological measurements of the transgenic seedlings indicated reduced oxidative damage,as evidenced by lower malondialdehyde (MDA) accumulation,increased superoxide dismutase (SOD) activity,and reduced hydrogen peroxide (H2O2) levels under salt stress,demonstrating enhanced salt tolerance.These findings suggest that SiLTP1 plays a positive role in regu-lating salt tolerance in foxtail millet,potentially by mitigating oxidative stress.This study provides a theoretical foundation and valuable genetic resources for the future development and breeding of salt-tolerant foxtail millet varieties.
lipid transfer proteinsSiLTP1salt stressfoxtail milletsalt tolerance response
万方数据
维普
