小麦面粉的颜色是其品质分级的重要指标,为了使小麦面粉的品质得到进一步提升,本研究利用位于TaPod-A1、TaPod-A3和TaPod-D1位点基因的功能标记对110份新疆小麦品种(系)进行等位变异基因检测.基因型与表型方差分析结果显示,TaPod-A1、TaPod-A3和TaPod-D1位点的等位变异类型TaPod-A1b(35.5％)、TaPod-A3c(53.6％)和TaPod-D1b(60％)较其他等位变异类型TaPod-A1a(64.5％)、TaPod-A3a(46.4％)和TaPod-D1a(40％)均具有较高的POD活性.在新疆小麦材料中,TaPod-A1、TaPod-A3和TaPod-D1的3个不同基因位点上高POD活性的优异等位变异类型的分布频率均表现为引进品种(系)≈自育品种(系)>地方品种.TaPod-A3b在110份新疆小麦材料中并未检测出,说明该等位变异为稀有等位变异.具有TaPod-A1、TaPod-A3和TaPod-D1基因等位变异组合品种(系)的平均POD活性高低依次为TaPod-A1b/TaPod-A3c/TaPod-D1b(2836.25 U g-1 min-1)>TaPod-A1b/TaPod-A3c/TaPod-D1a(2796.00 U g-1min-1)>TaPod-A1b/TaPod-A3a/TaPod-D1b(2520.31 U g-1 min-1)>TaPod-A1a/TaPod-A3c/TaPod-D1b(2473.91 U g-1 min-1)>TaPod-A1a/TaPod-A3a/TaPod-D1b(2407.65 U g-1 min-1)>TaPod-A1b/TaPod-A3a/TaPod-D1a(2339.06 U g-1 min-1)>TaPod-A1a/TaPod-A3c/TaPod-D1a(2320.38 U g-1 min-1)>TaPod-A1a/TaPod-A3a/TaPod-D1a(2210.69 U g-1 min-1).其中,具有TaPod-A1b/TaPod-A3c/TaPod-D1b等位变异基因的活性(2836.25 U g-1 min-1)极显著高于具有TaPod-A1a/TaPod-A3a/TaPod-D1a(2210.69 U g-1 min-1)(P<0.01)的品种,说明具有较多优异等位变异类型的品种具有较高的POD活性.
Allelic variation and distribution of peroxidase activity genes TaPod-A1,TaPod-A3,and TaPod-D1 of wheat in Xinjiang,China
To further improve the quality of wheat flour,functional markers for the TaPod-A1,TaPod-A3,and TaPod-D1 loci were employed to detect allelic variations in 110 wheat varieties (lines) from Xinjiang.Genotypic and phenotypic ANOVA results re-vealed that the TaPod-A1b (35.5％),TaPod-A3c (53.6％),and TaPod-D1b (60％) alleles associated with significantly higher POD activity compared to TaPod-A1a (64.5％),TaPod-A3a (46.4％),and TaPod-D1a (40％) alleles,respectively.In Xinjiang wheat cultivars,the distribution frequencies of favorable high-POD activity alleles at the TaPod-A1,TaPod-A3,and TaPod-D1 loci were higher in introduced varieties (lines)≈self-fertile varieties (lines) compared to landraces.Notably,the TaPod-A3b allele was absent in all 110 Xinjiang wheat samples,suggesting that it is a rare allele.The average POD activity of cultivars (lines) with specific alleles at the TaPod-A1,TaPod-A3,and TaPod-D1 genes followed the order:TaPod-A1b/TaPod-A3c/TaPod-D1b (2836.25 U g-1 min-1)>TaPod-A1b/TaPod-A3c/TaPod-D1a (2796.00 U g-1 min-1)>TaPod-A1b/TaPod-A3a/TaPod-D1b (2520.31 U g-1 min-1)>TaPod-A1a/TaPod-A3c/TaPod-D1b (2473.91 U g-1 min-1)>TaPod-A1a/TaPod-A3a/TaPod-D1b (2407.65 U g-1 min-1)>Ta-Pod-A1b/TaPod-A3a/TaPod-D1a (2339.06 U g-1 min-1)>TaPod-A1a/TaPod-A3c/TaPod-D1a (2320.38 U g-1 min-1)>Ta-Pod-A1a/TaPod-A3a/TaPod-D1a (2210.69 U g-1 min-1).Among these,the allele combination TaPod-A1b/TaPod-A3c/TaPod-D1b exhibited significantly higher POD activity (2836.25 U g-1 min-1) compared to the combination TaPod-A1a/TaPod-A3a/TaPod-D1a (2210.69 U g-1 min-1) (P<0.01),indicating that varieties with superior allele combinations exhibit enhanced POD activity.
万方数据
维普
