Identification and genome-wide association study of specialized seedling grass barley cultivars
Seedling grass factories represent an innovative strategy to ensure a consistent year-round forage supply for herbivores.This study was conducted to assess the conversion efficiency of seedling grass and to identify loci governing biomass in a diverse collection of 124 Chinese barley breeding varieties and germplasm.Our results indicate that following barley seed germination,the biomass of seedling grass in hydroponic systems undergoes exponential growth,leveling off after seven days.Within the plant factory setting,we have pinpointed ten varieties with superior conversion rate for seedling grass,including Dongqing 16 and Zaqing 6.A negative correlation was detected between the biomass of seedling grass and the thousand-kernel weight of the seeds.Genome-wide association studies revealed twelve QTL loci linked to seedling grass biomass,and eight candidate genes impli-cated in biomass regulation were predicted.This research not only identifies high-conversion varieties suitable for production in barley seedling grass factories but also lays the groundwork for genetically enhancing barley varieties specifically for seedling grass production.
barley seedling grassbiomass regulatory locigenome-wide association study