Identification of the R2R3-MYB transcription factor family and screening of genes regulating flavonoid synthesis in mung bean
The R2R3-MYB transcription factor family plays a crucial regulatory role in the synthesis of secondary metabolites,stress responses,and the growth and development of plants.This study employed bioinformatics analysis to identify the R2R3-MYB transcription factors across the entire genome of mung bean (Vigna radiata L.),and to predict their physicochemical properties,phylogenetic evolution,chromosome localization,cis-acting elements of the promoter,and gene structure.The expres-sion patterns of mung bean under different tissues,exogenous hormones,and stress conditions were analyzed using transcriptome data and Quantitative Real-time PCR (RT-PCR).R2R3-MYB members potentially involved in regulating the biosynthesis of mung bean flavonoids were screened through correlation analysis and protein interaction networks.The results demonstrated that a total of 168 R2R3-MYB members were identified in mung bean,145 of which were distributed across 11 chromosomes,while the chromosome information for 23 members remains unknown.Most of these members contain three exons encoding proteins ranging from 99 to 1645 amino acids and are all hydrophilic.The phylogenetic analysis divided the R2R3-MYB gene family in mung beans into 30 subgroups (V1-V30),revealing structural differences among members of different subgroups.Collinearity analysis within the mung bean genome indicated that all segmental duplication events underwent purifying selection.Analysis of cis-acting elements in the promoter regions of the R2R3-MYB genes in mung beans revealed a large number of hor-mone-responsive and stress-responsive elements,as well as a small number of flavonoid synthesis-responsive elements.Gene expression analysis showed that members with higher expression levels in leaves,petioles,hypocotyls,and seed coats accounted for 15.5％,16.1％,16.1％,and 10.7％ of the total,respectively.RT-PCR analysis indicated that the relative expression levels of almost all R2R3-MYB family members significantly decreased under low-temperature stress,with different members had diverse response patterns to stress.Protein interaction and correlation analysis suggested that the genes VrMYB6,VrMYB77,and VrMYB93 may be involved in the regulation of flavonoid biosynthesis in mung beans.The results of this study lay the foundation for further in-depth research on the function of the R2R3-MYB transcription factor family in mung beans.
mung beanR2R3-MYB transcription factortranscription factor family analysisflavonoids synthesis regulatory genes
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