Laccase is a family of copper-containing polyphenol oxidases primarily involved in lignin synthesis and resistance to various stresses in plants.In this study,members of the laccase gene family (BnaLACs) in Brassica napus were identified,and their physical and chemical properties were measured,including the number of amino acids,molecular weight,isoelectric point,instability index,and aliphatic index.The chromosome positions,evolutionary relationships,gene structures,and tissue expression patterns of these genes were subsequently predicted and analyzed.The results showed that the Brassica napus genome contains 53 BnaLAC family members,which are generally alkaline and stable proteins.Most BnaLACs are located in the vacuole membrane and outside the cell.Gene structure analysis revealed that BnaLACs have conserved structures.Tissue expression pattern analysis indicated that BnaLACs are expressed in all tissues except anthers,with higher expression levels in roots,seeds,silique walls,and stems.The ex-pression pattern of BnaLAC4s in stems was specifically analyzed,and it was found that BnaA05G0074200ZS is significantly corre-lated with lodging resistance in Brassica napus.Haplotype analysis showed significant differences in lodging resistance and lignin content between different BnaA05G0074200ZS haplotype lines.The results of this study provide a foundation for further analysis of the functions of the laccase gene family in Brassica napus and the mechanisms underlying stem lodging resistance.
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