Response of transcription factor StFBH3 under abiotic stress in potato
The basic helix-loop-helix (bHLH) transcription factor family is the second largest family of transcription factors and plays a central regulatory role in enabling plants to defend against environmental stress.Investigating the function of bHLH family genes in potato can provide a theoretical foundation for potato improvement and breeding.In this study,the StFBH3 gene was cloned,and its tissue-specific expression patterns under different stress treatments were analyzed using qPCR.The relative expression level of StFBH3 was highest in potato roots and leaves,and its expression was induced by osmotic stress,high salinity,and abscisic acid (ABA) treatment.On MS medium containing various concentrations of mannitol,NaCl,and ABA,the chloro-phyll content in the StFBH3 overexpressing potato lines was significantly higher than that of the wild type,and the root length was notably longer.Under drought and high salinity conditions,the StFBH3 overexpressing lines cultivated in soil exhibited stronger tolerance compared to the wild type,with significantly higher leaf relative water content,chlorophyll content,and super-oxide dismutase (SOD) enzyme activity.Furthermore,qPCR analysis revealed that the expression levels of drought-and salt stress-related genes,such as KAT1,were significantly reduced in the StFBH3 overexpressing lines compared to the wild type.These results suggest that StFBH3 functions as a positive regulator,induced and specifically expressed in response to osmotic stress,drought,and high salinity.This study provides an important reference for further analysis of the biological function of the StFBH3 gene in potato.
万方数据
维普
