QTL mapping of tiller angle in qingke (Hordeum vulgare L.)
Tiller angle (TA) is a crucial component of qingke (hulless barley) architecture,significantly influencing lodging resistance and grain yield.To investigate the genetic basis of TA,we constructed a high-density genetic linkage map using reduced-representation genome sequencing on recombinant inbred lines (RILs) developed from two parental lines:'Dazhangzi' (characterized by a loose plant architecture) and 'Kunlun 10' (characterized by a compact plant architecture).Quantitative trait locus (QTL) mapping was performed based on phenotypic data collected from multiple environments.Additionally,residual hybrid lines (RHLs) derived from the RILs were used to expand the population and fine-map the major QTL,qTA7H-1.A total of nine QTLs associated with TA were identified across seven chromosomes in qingke,with phenotypic variation explained (PVE) ranging from 6.41％ to 33.57％.Two QTLs,qTA3H-1 and qTA7H-1,were consistently detected across various environmental conditions,showing average additive effects of 5.42° (increasing TA) and-3.87° (decreasing TA),respectively.Four RHLs were selected within the initial localization interval of qTA7H-1,and F8:9 near-isogenic lines (NILs) were developed through self-pollination.To further refine the mapping,four-teen pairs of molecular markers were designed and densely placed within the QTL's confidence interval,targeting the extreme single lines of the RHLs.Ultimately,qTA7H-1 was fine-mapped to a 9.54 Mb physical interval between markers PC08 (32,252,397) and PA10 (41,790,765) using five types of recombinant individuals.Taken together,this study elucidates the genetic factors controlling TA,provid-ing a foundation for genetic improvement and molecular breeding of qingke with optimized architecture.
万方数据
维普
