Isolation and purification of antimicrobial substance produced by endophytic bacteria Y13 in the Camellia and its inhibitory effect on Camellia anthracnose
Bacillus subtilis strain Y13 isolated from Camellia has strong inhibitory activity against Colletotrichum gloeosporioides.In order to identify the antimicrobial compounds and the inhibition mechanism against C.gloeosporioides,the antimicrobial substances were isolated and purified by ethanol precipitation,solid phase extraction and reversed phase high performance liquid chromatography.Antifungal activity assay indicated that 16 main peak fractions had inhibition activity.The 16 main peak fractions can be divided in two groups.One group at retention time from 10.5 min to 26.0 min showed inhibition activity.The peak fraction at retention time 11.0 min showed the strongest activity and the molecular weight was 1 042.56 u identified by LC-MS.The other group at retention time from 27.5 min to 39.5 min dark the mycelia of C.gloeosporioides.The compound at retention time 37.5 min showed the strongest activity and the molecular weight was 1 480.85 u.Microscope observation revealed that the 2 compounds could break up and enlarge the mycelia,deform and swell the spores,and inhibit the spore germination.
Camellia anthracnoseantagonistic bacteriaantimicrobial substanceisolation and purificationinhibition mechanism
NETL
NSTL
万方数据
维普
