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甘蔗线条花叶病毒HC-Pro基因的分子变异分析

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为了解析甘蔗线条花叶病毒Sugarcane streak mosaic virus (SCSMV)不同分离物HC-Pro基因的分子变异规律,本研究利用RT-PCR法扩增获得SCSMV HC-Pro基因的序列,通过生物信息学分析,分别从重组、系统发生、选择压力等方面研究SCSMV HC-Pro基因的分子变异特征.共测定了44条SCSMV HC-Pro基因序列,相似性最低值为70%;HC-Pro基因重组频率较低,仅发现3个重组位点,其中一个系首次报道;与先前报道相比,部分新测定云南蔗区的SCSMV分离物在HC-Pro基因上形成一个新组—第Ⅲ组;HC-Pro基因处于很强的负选择压力作用,未发现正向选择作用位点.本研究结果进一步证明SCSMV HC-Pro基因具有高度的遗传多样性.
Molecular variation of HC-Pro gene of Sugarcane streak mosaic virus
The objective of this study is to assess the molecular evolution and divergence of SCSMV according to HC-Pro gene sequences.The HC-Pro gene sequences of SCSMV were obtained by RT-PCR,and analyzed by bioinformatic software,in aspect of recombination,phylogenetics,selection,demography,and gene flow.In the present study,44 HC-Pro gene sequences were determined with a 70% lowest similarity;only one novel recombination site together with two previous reported sites were found in HC-Pro gene,suggesting that infrequent recombination events were distributed in this gene of SCSMV;one novel lineage (lineage Ⅲ) clustered by SCSMV sequences determined here was found;strong purifying selection was found in the HC-Pro gene of SCSMV.Our genetic study further indicates that the HC-Pro gene showed high genetic diversity in the genome of SCSMV.

Sugarcane streak mosaic virusHC-Promolecular variation

贺振、李文凤、张志想、李世访

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扬州大学园艺与植物保护学院,扬州225009

中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193

云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,开远661699

甘蔗线条花叶病毒 HC-Pro基因 分子变异

植物病虫害生物学国家重点实验室开放课题扬州大学科技创新培育基金

SKLOF2015182015CXJ043

2017

植物保护
中国植物保护学会 中国农业科学院植物保护研究所

植物保护

CSTPCDCSCD北大核心
影响因子:1.004
ISSN:0529-1542
年,卷(期):2017.43(2)
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