Dynamic change of endogenous hormones during the formation of haustorium of Orobanche aegyptiaca parasitizing melon
In this study,the formation process of haustorium of Orobanche aegyptiaca was analyzed by paraffin section and microscopic observation,and the dynamic changes of endogenous hormones in O.aegyptiaca at different stages of haustorium formation during parasitism to melon roots were detected by liquid chromatography-mass spectrometry(LC-MS).The results show that the seeds of O.aegyptiaca began to germinate on the third day after inoculation,the haustorium top was firmly attached to the melon root on the 7th day,and then haustorial cell invaded the cortex of melon root,forming a large number of claw-like structures in the cell layer of the cortex of melon root.On the 11th day,haustorial cells were connected with host vascular bundle.Phytohormone test results showed that a total of 17 phytohormones were involved in the formation process of haustorium in O.aegyptiaca,and the contents of auxin,cytokinin and brassinolide changed significantly.The content of auxin indole-3-carboxaldehyde decreased by 83.36％,the content of cytokinin-like trans-zeatin-riboside increased by 5.02 times and brassinolide increased by 481.95 times in the formation process of haustorium.These three phytohormones might play an important role in regulating haustorium formation in O.aegyptiaca.
万方数据
维普
