Identification and primary inoculum sources of root-knot nematode in Glycine max in Fujian province
A serious root-knot nematode disease occurring at the seedling stage of Glycine max'maodou 64',with a disease incidence of 78％,has been found in fields in Zhangzhou region,Fujian province.Morphological measurements of females,males and second juveniles(J2s)matched the original description of Meloidogyne graminicola.Species identification was further carried out by phylogenetic analysis based on the sequences of rDNA-internal transcribed spacer(ITS)region and D2D3 region of 28S rDNA.The inoculation test in greenhouses confirmed the nematode's pathogenicity.This is the first report of M.graminicola parasitizing soybean in Fujian province.A large number of J2s had been isolated from the root of preceding rice stubbles and rhizosphere soil in ridged fields of the'maodou 64',and the species identification was confirmed with M.graminicola specific primers.These results indicated that the root of the preceding rice was infected by M.graminicola,and eggs and J2s of the nematode survived in waterlogged soil and infected root for a long period of time in irrigated rice cultivation,and became the primary inoculum sources for the root-knot nematode disease when soybeans are planted as a rotating crop.
万方数据
维普
