Detection and identification of viruses and viroids in Prunus mume Siebold & Zucc in China
The occurrence and distribution of viruses and viroids infecting Prunus mume Siebold & Zucc.in China remain partially understood.In this study,we collected 64 suspected leaf samples infected by virus from P.mume gardens in Beijing,Wuhan,Nanjing and Wuxi,and performed detection of seven viruses and two viroids using RT-PCR and dot-blot hybridization.Six virus species and one viroid species were detected from these samples.Among them,prunus necrotic ringspot virus(PNRSV)and peach latent mosaic viroid(PLMVd)were first identified in P.mume in China.PNRSV,Asian prunus virus 2(APV2),and peach leaf pitting-associated virus(PLPaV)had a high infection rate of more than 30％.Based on geographic distribution and detection rates,PLPaV,APV2,PNRSV and plum bark necrosis stem pitting-associated virus(PBNSPaV)should be the main viruses in P.mume in Wuhan,Nanjing,and Wuxi.Furthermore,the nucleotide sequences of PLMVd and mume virus A(MuVA)genomes,RNA1 of PLPaV,and coat protein(CP)of PNRSV were obtained by cloning and sequencing.The results of sequence alignment showed that Chinese isolates of PLMVd and PNRSV were individually closest to their corresponding Chinese peach isolates,suggesting possible transmission between P.mume and peach trees.Moreover,Chinese P.mume isolate of MuVA had the highest similarity with Japanese isolate.Chinese isolate of PLPaV markedly differed from Chinese peach isolate.These findings further clarify the occurrence and distribution of viruses and viroids in P.mume in China,and are helpful for understanding the epidemic and transmission of these viruses and viroids.
万方数据
维普
