Screening for the resistant varieties of Asparagus officinalis against Bemisia tabaci and the mechanism of resistance
To explore the mechanisms underlying the resistance of asparagus to Bemisia tabaci(Gennadius)and provide a theoretical basis for breeding resistant asparagus varieties and develop comprehensive prevention and control methods for B.tabaci,16 asparagus varieties were collected and inoculated with B.tabaci at the seedling stage to determine the contents of chlorophyll a,chlorophyll b,peroxidase,superoxide dismutase,soluble protein,soluble sugar,free proline,malondialdehyde,tannin,flavonoids,total phenols,and cuticular waxes as the index for resistance evaluation.The resistance of 16 asparagus varieties and the related mechanisms were investigated by using membership function method,insect ratio method and phenotypic observation.The results showed that there were significant differences in the number of B.tabaci among different varieties under different treatments,and different asparagus varieties had different attraction ability to B.tabaci.The biochemical indexes varied greatly after damaged by B.tabaci,and the variation coefficient ranged from 24.46％to 47.43％.Among the 16 asparagus varieties,'Zhefeng 1''Atlas'and'Purple passion'had relatively strong resistance to the pest insect.The cumulative contribution rate of five principal components of 12 biochemical indexes was 84.136％.The correlation analysis showed that the resistance of asparagus to B.tabaci was extremely significantly positively correlated with tannin and cuticular waxes,significantly positively correlated with activity of POD,contents of total phenols and soluble sugar,extremely significantly negatively correlated with malondialdehyde,while no significant correlation with other traits was observed.Asparagus varieties with high contents of tannin,cuticular wax,soluble sugar and total phenols,high POD activity,and low content of malondialdehyde should be paid more attention in resistance-breeding of asparagus to B.tabaci.
万方数据
维普
