摘要
番茄斑萎病毒(tomato spotted wilt virus,TSWV)和烟草花叶病毒(tobacco mosaic virus,TMV)是2种重要的植物病原病毒,对多种经济作物的产量和品质均造成严重影响.2021年-2022年,在云南省丽江市烟草种植区不同烟区采集叶片黄化、皱缩以及无症状的青蒿Artemisia caruifolia样品共计14份,利用免疫金标速测卡和RT-PCR对其病原病毒进行检测.利用免疫金标速测卡检测结果显示,在所检样品中有9份样品检测出TSWV,检出率为64.28％,有3份样品检测出TMV,检出率为21.43％,2种病毒复合侵染的检出率同样为21.43％;利用RT-PCR对复合侵染的3份样品进行分子检测,结果显示,在3份复合侵染青蒿样品中获得3条TSWVN基因序列、3条TMV cp基因序列和2条TMV RdRp部分序列.TSWV青蒿分离物与分离自云南的TSWV-2分离物相似性最高,为99.6％;TMV青蒿分离物与分离自辽宁的TMV-Shenyang分离物和分离自云南的TMV-Yongren-1相似性最高,均大于99.4％.这是首次发现TSWV和TMV 2种不同属病毒复合侵染青蒿.
Abstract
Tomato spotted wilt virus(TSWV)and tobacco mosaic virus(TMV)are two important viral pathogens that account for significant yield losses and quality damage in many economically important crops.During 2021-2022,14 sweet wormwood(Artemisia caruifolia)leaf samples with leaf yellowing and curling symptoms or asymptomatic were collected from tobacco fields in Lijiang of Yunnan province.The colloidal gold antigen rapid screen kits and RT-PCR were used to identify the pathogens of diseased plants.Immunological assays using colloidal gold antigen rapid screen kits showed that nine leaf samples were infected with TSWV(64.28％positive rate),three infected with TMV(21.43％positive rate),and three infected with both TSWV and TMV.RT-PCR was carried out to obtain viral sequences from these three co-infected sweet wormwood samples.Then three N gene sequences of TSWV,three cp sequences and two partial RdRp sequences of TMV were obtained.Sequence alignment showed that the TSWV isolates were most closely related to the TSWV-2 isolate from Yunnan sharing 99.6％sequence identity,and the TMV isolates shared the highest sequence identity(＞99.4％)with the Shenyang isolate from Liaoning and Yongren-1 isolate from Yunnan.It is the first report that sweet wormwood plants were co-infected by TSWV and TMV.
基金项目
中国烟草总公司云南省公司科技计划(2021530000242016)
云南省种子种业联合实验室项目(202205AR070001-02)
国家自然科学基金(32160619)
维普
万方数据