Cloning and expression profiling of ATP-binding cassette transporter G1 gene in grain aphid Sitobion miscanthi
Grain aphid Sitobion miscanthi is a migratory pest as well as the dominant pest on wheat in China.Molting hormone,ecdysone(20E),as one of the internal hormone,involved in the regulation of wing differentiation of aphids,which induces a higher proportion of wingless aphid in the offspring and maintains a high titer in winged adults.To enter and exit the target cells,20E requires the transport of specific membrane transporters.ABCG1,as one of 20E membrane transporters in insects,negatively regulates ecdysone signaling by transmembrane transport of insect ecdysone,which has not been reported in aphids yet.In the present study,SmisABCG1 was cloned and aligned with its orthologs,and their phylogenetic relationship was analyzed among insects.Expression patterns among organs or tissues of both winged and wingless adults,pseudo embryos,first to fourth instar nymphs were also analyzed.The results showed that the complete open reading frame of SmisABCG1 was 1 851 bp,encoding 616 amino acids,with seven trans-membrane regions,which was consistent with the traits of ABCG protein family member.The gene accession ID was OP626323.ABCG1 is conserved among insects,and the phylogenetic relationship of this protein confirmed that there is a close genetic relationship among species.SmisABCG1 shared high consistency(＞87％)of sequence with ABCG1s in other insects such as Acyrthosiphon pisum,Rhopalosiphum padi,Aphis gossypii,A.craccivora,Cinara cedri,and these aphids clustered into a branch.The closest neighbor of SmisABCG1 is ApisABCG1 in A.pisum,followed by white-backed planthopper(Sogatella furcifera),plant hopper(Nilaparvata lugens),small brown planthopper(Laodelphax striatellus),sawfly(Athalia rosae),alishan dive fly cocoon bee(Fopius arisanus),red flour beetle(Tribolium castaneum)and Aethina tumida.RT-qPCR results showed that SmisABCG1 is mainly expressed in pseudo embryo as well as adults.Significantly high level of pseudo embryo in winged adult and equal level of winged and wingless adults with embryo indicated that wingless aphid expressed higher ABCG1 than winged aphid.Subsequent analysis found that this was due to a significantly higher expression in the thorax of wingless adult.All other organs or tissues showed no significant differences between winged and wingless phenotype.Based on the negative regulation of ecdysone by this protein,it consistent with low transcript levels but high ecdysone levels in winged adult aphids.
万方数据
维普
