摘要
为了解硝普钠是否可以缓解除草剂烯禾啶对谷子的药害,本试验以谷子优质品种'晋谷21号'愈伤组织为试验材料,将不同浓度硝普钠与烯禾啶复配后添加到培养基中对其进行胁迫处理,观察不同处理下愈伤组织的生长情况,并分别测定其抗氧化酶活性,渗透调节物质含量等相关生理指标,通过分析比较这些指标在不同处理间的差异,了解硝普钠对烯禾啶药害的缓解效应,并对其解毒机制进行初步探讨.结果表明,硝普钠对谷子烯禾啶药害有一定缓解作用,且缓解作用随烯禾啶处理浓度而发生变化,硝普钠浓度需根据烯禾啶浓度进行调整.硝普钠与烯禾啶复配提高了愈伤组织细胞抗氧化酶活性和渗透调节物质含量,减轻烯禾啶对谷子的损伤.本研究为生产中使用硝普钠减轻谷子烯禾啶药害提供了一定的理论依据.
Abstract
To investigate whether sodium nitroprusside can alleviate the harm of the herbicide sethoxydim to foxtail millet,calli from the high-quality foxtail millet cultivar'Jingu 21'were used as experimental materials.The callus was treated with different concentrations of sodium nitroprusside and sethoxydim.The growth of the callus was observed,and the antioxidant enzyme activity and osmotic adjustment substance content were measured under different treatments.Differences in these indexes were analyzed and compared to understand the effect of sodium nitroprusside in alleviating the harm caused by sethoxydim and to explore its detoxification mechanism.The results showed that sodium nitroprusside could alleviate the harm of sethoxydim to foxtail millet,and the appropriate concentration of sodium nitroprusside should be adjusted according to sethoxydim concentration.The sodium nitroprusside mixed with sethoxydim increased the activity of antioxidant enzymes and the content of osmotic-regulating substances in the callus of foxtail millet,and alleviated the damage of sethoxydim to foxtail millet.It provides a theoretical basis for the use of sodium nitroprusside in foxtail millet production to reduce the damage caused by sethoxydim.
基金项目
现代农业产业技术体系建设项目(CARS-06-14.5-A28)
山西省谷子产业技术体系建设项目(GZTX202215)
山西省回国留学人员科研项目(2020-068)
NETL
NSTL
万方数据