Gene cloning and expression analysis of coronins in Laodelphax striatellus
The coronins of Laodelphax striatellus are the members of WD40-repeat protein superfamily,which can regulate the dynamic polymerization and depolymerization of actin cytoskeleton.In this study,we cloned the coding sequences of three coronins:coronin 1A,coronin 2B,and coronin 7 of L.striatellus by RT-PCR.Phylogenetic analysis showed that these three coronins belonged to separate branches,with the highest homology observed with Nilaparvata lugens,showing amino acid sequence similarities of 99.2％,88.2％and 82.5％,respectively.Analysis of transcription levels of coronin genes showed that transcription level of coronin 1A in adults was significantly higher than that in the 2nd-and 4th-instar nymphs,transcription level of coronin 2B in male adults was significantly higher than in the 2nd-and 4th-instar nymphs and female adults,and transcription level of coronin 7 in female adults was significantly higher than that in the 2nd-and 4th-instar nymphs and male adults.Moreover,the transcription level of the three coronin genes in the salivary glands were significantly higher than those in the gut.In addition,the transcription levels of the three coronin genes in L.striatellus carrying rice stripe tenuivirus(RSV)were significantly higher than in non-viruliferous insects,indicating that the three coronins may play a role in transmitting RSV.This study have laid a foundation for further studies on the function of coronins in virus-transmission.
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