Survey and genetic diversity analysis of chilli veinal mottle virus in tobacco-producing areas of Yunnan province
The objective of this study is to clarify the distribution,genetic diversity,and population genetic structure of chilli veinal mottle virus(ChiVMV)in tobacco production areas across six prefectures(cities)in Yunnan province.It holds great significance for disease prevention,control,and early warning measures.In 2022,96 tobacco samples suspected of ChiVMV infection were collected from different tobacco production areas in these prefectures(cities).The tobacco samples were detected by electron microscope negative staining observation and molecular biological methods.The cp gene sequences were obtained from 21 ChiVMV Yunnan tobacco isolates.The phylogenetic evolution,genetic diversity and population genetic structure characteristics of the cp gene sequences from the 21 Yunnan tobacco isolates and 38 ChiVMV isolates reported on NCBI,originating from different countries,regions,and hosts,were analyzed using biological software such as SDT,MEGA,RDP,DnaSP,and Arlequin.The results showed that the positive rate of ChiVMV was 51.04％among the 96 tobacco samples collected from the six prefectures(cities)in Yunnan.Sequence alignment revealed nucleotide consistency of the cp gene above 84.1％,with 38 ChiVMV isolates reported on NCBI.Phylogenetic analysis showed that 59 ChiVMV isolates from different sources were categorized into four branches.The clustering results exhibited obvious geographical distribution characteristics unrelated to host plants.Genetic diversity and genetic differentiation analysis revealed high genetic diversity among the four geographical populations of ChiVMV cp gene.Evident genetic differentiation had occurred between the Chinese population and populations from India,Thailand and other countries,with statistically significant differences(P＜0.05).Heritability analysis suggested that gene exchange,genetic drift,and negative selection of genes were important adaptive evolution mechanisms for ChiVMV isolates.
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NSTL
万方数据
