Construction of genetic transformation system of Fusarium commune causing lotus rhizome rot
Lotus rhizome rot caused by Fusarium commune is one of the major diseases in lotus production in China.In this study,the strongly pathogenic strain FCN23 was used as the target strain,and the effects of enzymatic digestion time,mycelial age and osmotic stabilizer on the protoplast preparation were investigated by PEG-mediated genetic transformation.When the conidia were cultured in YPD liquid medium for 24 h to obtain fresh mycelium,the enzyme digestion time was 2.5 h,the osmotic stabilizer was 0.7 mol/L NaCl solution,and the efficiency was the highest.Geneticin resistance gene and green fluorescent protein gene were transfered into the protoplast of F.commune.The results showed that stably expressed transformants were obtained,which were able to stably inherit the gfp gene,indicating that the genetic transformation system of F.commune was successfully constructed.The transformation system laid the foundation for the study of the infestation process and gene function of F.commune.
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