Determination of glufosinate-ammonium,glyphosate and their metabolites in soil and water by HPLC-MS/MS
An ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)combined with solid-phase extraction purification was used to establish a multi-residue analysis method for the simultaneous determination of glyphosate,glufosinate-ammonium and their six metabolites in water or soil.Oasis MCX and Oasis MAX solid phase extraction columns were used in pre-treatment,and then 2％formic acid methanol water(volume ratio of 1∶1)was used for elution.The eluent collected was evaporated to dryness by a rotary evaporator and diluted with 0.1％ammonia.The linear range of the method was 0.02-0.5 mg/L.Under the spiked level of 0.000 1 and 0.001 mg/L in the purified water,the recoveries were 72.8％-94.2％and the relative standard deviations were 2.3％-16.1％,respectively.Under the spiked level of 0.001 mg/kg and 0.01 mg/kg in the soil,the recoveries were 70.6％-88.5％and the relative standard deviations were 3.6％-12.8％,respectively.The method is accurate,sensitive and comprehensive,which could be used to analyze the content of glyphosate,glufosinate-ammonium and their metabolites in low concentration samples in environmental test.
water and soilglufosinate-ammoniumglyphosatemetaboliteUHPLC-MS/MSresidue analysis
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