Characterization of the subcellular localization and pathogenicity of cucurbit chlorotic yellows virus coat protein
Cucurbit chlorotic yellow virus(CCYV)is a kind of plant virus transmitted by whitefly.Recently,it has caused serious losses in the production of Cucurbitaceae crops.In order to study the subcellular localization and pathogenic characteristics of its coat protein(CP)during infection,the fluorescence vector CP-YFP and the heterologous expression vector pGR-CP were constructed based on CCYV Shandong isolate.After infiltrating the vector CP-YFP for 48 h,fluorescence signal could be observed in cytoplasm and nucleus of the Nicotiana benthamiana leaves under the laser confocal microscope.After seven days of infiltrating the vector pGR-CP,the upper leaves of N.benthamiana plants began to show mosaic symptoms,and 13 days later,the systemic leaves appeared severe chlorotic,crumple and necrotic spots.These results indicated that CP might be an important protein involved in the pathogenicity of CCYV.This study provided a preliminary theoretical support for further understanding the pathogenesis of this virus.
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