Molecular identification and analysis of tomato spotted wilt virus on eggplant in Beijing
In this study,serious viral disease was found on Solanum melongena L.in a greenhouse in Beijing.With high-throughput sequencing technology based on small RNAs and RT-PCR methods,it was confirmed that the eggplants were infected by tomato spotted wilt virus and named as TSWV-eggplant isolate.Complete genome sequence of the TSWV S RNA,M RNA,L RNA was further cloned and the phylogenetic tree was also constructed.The results showed that the S RNA of TSWV-eggplant was closely related with isolates from America,M RNA was closely related with isolates from China,while L RNA was closely related with isolates from Korea.So the TSWV isolate in this study was different from isolates reported in China and whether the genome of the isolate was recombined from different isolates was needed to be further studied.
NETL
NSTL
万方数据
