北京地区侵染茄子的番茄斑萎病毒分子鉴定与分析
Molecular identification and analysis of tomato spotted wilt virus on eggplant in Beijing
田文 1邱艳红 2秦文韬 3张海军 2王德欣 2李龙飞 4贾志杨 4徐秀兰2
作者信息
- 1. 北京市农林科学院蔬菜研究所,北京 100097;中国科学院微生物研究所,北京 100101
- 2. 北京市农林科学院蔬菜研究所,北京 100097
- 3. 北京市农林科学院植物保护研究所,北京 100097
- 4. 京研益农(北京)种业科技有限公司,北京 100097
- 折叠
摘要
调查发现北京地区一温室栽培茄子Solanum melongena L.出现严重病毒病.利用基于小RNA的高通量测序技术和RT-PCR方法,明确了引起茄子病害的病毒种类为番茄斑萎病毒,将其命名为TSWV-eggplant分离物.进一步克隆了该病毒的基因组全长(S RNA、M RNA、L RNA),并构建其系统发育树.结果表明,该分离物的S RNA与美国分离物亲缘关系较近,M RNA与中国分离物亲缘关系较近,而L RNA与韩国分离物亲缘关系较近.因此,本研究发现的TSWV分离物与国内已发生报道的分离物不同,该分离物是否存在不同分离物之间基因组的重组需要进一步研究.
Abstract
In this study,serious viral disease was found on Solanum melongena L.in a greenhouse in Beijing.With high-throughput sequencing technology based on small RNAs and RT-PCR methods,it was confirmed that the eggplants were infected by tomato spotted wilt virus and named as TSWV-eggplant isolate.Complete genome sequence of the TSWV S RNA,M RNA,L RNA was further cloned and the phylogenetic tree was also constructed.The results showed that the S RNA of TSWV-eggplant was closely related with isolates from America,M RNA was closely related with isolates from China,while L RNA was closely related with isolates from Korea.So the TSWV isolate in this study was different from isolates reported in China and whether the genome of the isolate was recombined from different isolates was needed to be further studied.
关键词
番茄斑萎病毒/茄子/高通量测序Key words
tomato spotted wilt virus/eggplant/high-throughput sequencing引用本文复制引用
基金项目
北京市农林科学院科技创新能力建设专项(KJCX20230214)
北京市农林科学院科技创新能力建设专项(GYJC-202403)
北京市农林科学院青年科研基金(QNJJ202131)
出版年
2024
NETL
NSTL
万方数据