Control of Meloidogyne enterolobii by mixture of Purpureocillium lilacinum PLHN and reduction of emamectin benzoate 3％ME
Meloidogyne enterolobii is a serious threat to tropical agriculture.It is urgent to find a control method that combines the advantages of biological control,environmental friendliness,and efficient application of chemical pesticides.In this study,the nematocidal activity and resistance of biocontrol strain were measured.A series of compound combinations of nematicide and biocontrol strain were designed,and the optimal combination scheme to control M.enterolobii was selected.The results showed that emamectin benzoate 3％ME had little effect on mycelial growth,sporulation and conidial germination of Purpureocillium lilacinum PLHN.The LC50 and LC99 values of emamectin benzoate against M.enterolobii were only 0.107 mg/L and 0.792 mg/L,respectively,so the absolute lethal concentration was 0.8 mg/L.The inhibitory effects of 0.4 mg/L emamectin benzoate(50％reduction)+75％PLHN and 0.6 mg/L emamectin benzoate(25％reduction)+50％PLHN on the second-stage juveniles(J2)and eggs of the M.enterolobii were superior to those of other combinations.Pot experiment showed that the compound combination of 0.6 mg/L emamectin benzoate+50％PLHN had the best control effect and had a certain inhibitory effect on the formation of root-knot,which was equivalent to the absolute lethal concentration of emamectin benzoate,and could promote the growth of tomato.0.6 mg/L emamectin benzoate+25％PLHN and 0.4 mg/L emamectin benzoate+75％PLHN took the second place.The combination of emamectin benzoate and P.lilacinum PLHN not only reduced the dose of emamectin benzoate by 25％to 50％,but also made up for the weak stability of biological control and other deficiencies.It is an optional strategy for plant nematode disease control.
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