Molecular identification and genome sequence analysis of begomoviruses infecting Abutilon theophrasti
In order to clarify the pathogen and the genomic sequence characteristics that causing leaf wrinkling and mosaic of Abutilon theophrasti.The samples with typical symptoms were collected from Jinzhong area of Shanxi province,the genome sequence of the virus was amplified using geminivirus degenerate primers and then designed specific primers.The phylogenetic tree was constructed and the virus genomic sequence was analyzed using bioinformatics methods.The results showed that the pathogen causing the leaf wrinkling and mosaic symptoms of A.theophrasti was tomato yellow leaf curl virus(TYLCV),named TYLCV-Abu(GenBank acc.no.OP293347),but no beta satellite amplification was obtained.The viral DNA-A genome was 2 782 bp in length and contained six open reading frames.The TYLCV-Abu sequence shared the highest identity(98.99％)with that of the TYLCV eggplant isolate KSQ1-3(KC428753),but the proteins encoded by its C4 and V2 genes shared low identities with those of the other virus isolates in GenBank.The results of recombination analysis showed that the TYLCV-Abu isolate was recombined from the TYLCV-F isolate(KY971326)and the TYLCV-KSQ1-3 isolate.The recombination region was from 2 617 nt to 2 782 nt in TYLCV-Abu genome.To our knowledge,this is the first report of the whole genome sequence of TYLCV from A.theophrasti plants.
万方数据
维普
