Identification of the AiHSP 19.3 gene and its response to high temperature and insecticide stress in Agrotis ipsilon
The black cutworm,Agrotis ipsilon,is an important agricultural pest insect.Small heat shock proteins(sHSPs)are crucial for cellular defense against external stresses.This study aims to explore the response patterns of sHSP gene in different developmental stages of A.ipsilon and under high temperature and insecticide stresses.A cDNA sequence of sHSP,AiHSP 19.3,was identified from the A.ipsilon transcriptome using homology search and subsequently cloned by RT-PCR.The transcriptional pattern of the AiHSP19.3 gene in different developmental stages and tissues of A.ipsilon was analyzed using real-time quantitative PCR,along with transcript levels under high temperature and insecticide stresses.The results showed that the open reading frame of AiHSP19.3 was 516 bp,encoding a protein consisting of 171 amino acids with a molecular weight of 19.3 kD.AiHSP19.3 contained an α-crystallin domain(ACD),a hallmark feature of sHSP.Transcription of AiHSP19.3 was detected in all tested developmental stages and 3rd-instar larval tissues,with the highest level observed in the 6th-instar larvae and fat body of 3rd-instar larvae.Transcription levels of AiHSP19.3 significantly increased in larvae after treatment with high temperature and three insecticides(lambda-cyhalothrin,chlorantraniliprole,and phoxim),indicating that this gene may play an important role in responding to high temperature and insecticide stresses.
万方数据
维普
