The role of cell wall-degrading enzymes of Colletotrichum coccodes during its infection
To determine the function of cell wall-degrading enzymes(CWDEs)secreted by Colletotrichum coccodes during infecting potato stems,the types and activities of CWDEs secreted by C.coccodes both in vivo and in vitro were investigated,the gene expression levels of four polygalacturonases(PG)of C.coccodes were also assessed using RT-qPCR.The qualitative measurement results indicated that C.coccodes secretes pectinase,cellulase,and protease on detection plates in vitro,and the qualitative determination results demonstrated that it secretes carboxymethyl cellulase(Cx),β-glucosidase(β-Glu),polygalacturonase(PG),and polymethyl-galacturonase(PMG)on detection medium in vitro.In addition,C.coccodes produces PG,PMG and polygalacturonic acid trans-eliminase(PGTE)during its infection to potato stems in vivo,with peak activities of 26.50,77.61 U/mL and 0.16 U/mg 48 h,72 h and 144 h post-inoculation,respectively.Inoculating crude enzymes induced by sodium carboxymethylcellulose and pectin onto potato stems could cause potato stem rotting similar to the typical symptoms caused by C.coccodes on potato stems.Moreover,four PGs of C.coccodes showed significantly upregulated co-expression 24 h and 48 h post-inoculation.Collectively,these data demonstrated the important role of pectinase in the pathogenic process of C.coccodes.
万方数据
维普
