Identification of a biocontrol bacterium against potato dry rot and its antibacterial activity
Potato dry rot caused by Fusarium is one of the important diseases affecting both the yield and quality of potato during storage.Finding highly efficient and low-toxicity biocontrol agents to control potato dry rot holds great significance in improving the yield and quality of potato.In this study,the antifungal activity of strain 2-1-9-CJK-2 isolated from potato tubers against Fusarium solani was determined.The results revealed that various components of the strain,including the fermentation broth,sterile fermentation broth,bacterial suspension,intracellular components,and volatile organic compounds,exhibited notable antifungal activity against F.solani,with an inhibition rate of 93.37％,61.92％,80.82％,44.01％and 49.16％,respectively.A combination of morphological,physiological,and biochemical characteristics,along with multi-gene phylogenetic analysis,it was identified as Bacillus velezensis.Antifungal test using potato slice and tubers showed that the optimal control effect was achieved when the concentration of the fermentation broth was 1× 109 cfu/mL.In addition,the strain could secrete proteases,amylases,and cellulases,has along with the ability to dissolve phosphate and form biofilm,but it lacked hemolysis activity and displayed strong antifungal activity against six other pathogenic fungi.These results showed that B.velezensis 2-1-9-CJK-2 had a broad antifungal spectrum,biosafety,and strong antifungal activity against F.solani.Our study suggests its potential application in the prevention and control of plant diseases,thereby laying a foundation for the development of biocontrol agents for potato dry rot.
potato dry rotFusarium solaniBacillus velezensisbiological control
万方数据
维普
