Inhibition on postharvest fruit diseases by Meyerozyma caribbica and its degradation mechanism to patulin
To determine the inhibitory effects of the antagonistic yeast Meyerozyma caribbica on postharvest fruit diseases and the mechanism of toxin degradation,we studied the combined effect of M.caribbica and alginate oligosaccharide(AOS)on penicilliosis,gray mold,and black spot in strawberries,pears,apples,and tomatoes.The mechanism of patulin(PAT)degradation in vitro was also explored using different treatment solutions of antagonistic yeast.The results showed that M.caribbica at a concentration of 1×108 cfu/mL could effectively inhibit the incidence of penicilliosis in strawberries,pears,and apples,gray mold in strawberries,tomatoes,and grapes,and black spot in tomatoes,pears,and grapes.Adding 5 g/L AOS could significantly enhance the inhibitory effect of M.caribbica.The fermentation broth of M.caribbica effectively degraded PAT in apple wounds,with a degradation rate of 30.35％eight days after treatment and 39.15％after AOS induction.PAT(10 μg/mL)could be completely degraded by M.caribbica within 27 h in vitro.Our study further revealed that M.caribbica mainly degraded PAT through intracellular enzymes produced in the normal metabolic process of living cells,and the degradation ability of M caribbica was significantly enhanced after AOS induction,highlighting its potential as a biocontrol strain with enhanced capabilities.This study lays a foundation for the future application of this biocontrol strain.
fruitpathogenpatulinantagonistic yeastalgal oligosaccharidebiological control
万方数据
维普
