Study of glyphosate-resistant Eleusine indica in seed breeding base in Hainan
Eleusine indica is an annual malignant Poaceae weeds.This grass inflicts substantial damage to farmlands in the Huang-Huai-Hai Region and south of the Yangtze River.Glyphosate,renowned for its efficacy as a non-selective herbicide,is gradually finding application in corn,soybean,and other crop fields with the advancement of biological breeding industrialization.The emergence of glyphosate-resistant weeds in seed breeding bases serves as a potential factor for their rapid spread.To clarify the susceptibility of E.indica to glyphosate in Sanya,Hainan province,whole-plant bioassays,target gene mutation,and expression detection were conducted to detect the resistance levels and mechanisms.The results showed that the GR50 of this population was 2 053.0 g/hm2,with a resistance index(RI)of 5.0.There was no significant difference in the conserved region of EPSPS between the resistant and susceptible populations.However,the relative expression level of the target gene EPSPS in the resistant population was 47.4 times of that in the susceptible population.In addition,the EPSPS protein concentration in resistant plants was 17.1 times of those in susceptible plants.These results suggest the evolution of glyphosate resistance within the population,with one of the resistance mechanisms involving an increase in target protein content due to EPSPS overexpression.
万方数据
维普
