Reducing the use of chemical pesticides and improving the efficiency of biological pesticides in the field are crucial for reducing chemical pesticides reliance and ensuring the safety of agricultural products.In this study,a pot experiment was conducted using the tomato cultivar'Xinjinfeng 1'to investigate the control effect of fosthiazate combined with a biocontrol agent on tomato root-knot nematode and its effects on rhizosphere soil microorganisms by inoculation of root-node nematode.The results showed that the number of root-knot nematodes and gall index under 50％reduction of fosthiazate and mixed with 50％biocontrol agent significantly decreased compared to the control and single applications of fosthiazate or biocontrol agent.60 days post transplantation,the population of root-knot nematodes and gall index decreased by 49.61％and 32.50,respectively,resulting in an overall control efficacy of about 64.77％.Furthermore,the amount of culturable bacteria,actinobacteria,and total microorganisms in the rhizosphere of tomato plants increased by 76.21％,47.96％,and 69.60％under the combined application compared with single applications of fosthiazate.In addition,the relative abundance of Actinomycetota and Ascomycota increased significantly under combined application.In contrast,the relative abundance of Acidobacteriota and Bacillota showed an opposite trend.Similarly,the relative abundance of Patescibacteria,Planctomycetota,and Trichoderma significantly increased in the rhizosphere of tomato plants compared to single application of fosthiazate.However,single application of fosthiazate decreased the relative abundance of Trichoderma in the rhizosphere of tomato plants.In conclusion,50％reduction of fosthiazate combined with 50％biocontrol agent enhances the control efficacy on tomato root-knot nematodes by increasing the abundance of culturable microorganisms and beneficial microorganisms such as Actinomycetota and Trichoderma in the rhizosphere of tomato plants.
维普
万方数据