Identification of Bacillus strain JZB30-1 and its biological control effect on tomato grey mould
To obtain a Bacillus strain with significant control effect on tomato grey mould,antagonistic bacteria were screened by confrontation culture and two-layer plate methods,along with the detection of characteristics related to biocontrol.Its taxonomic status was identified based on morphological features,physiological-biochemical characteristics,and multiple gene sequences analyses.The disease-prevention and growth promotion effects of the obtained strain were evaluated by applying the fermentation filtrate of the strain to tomato fruits and potted tomato seedlings,respectively.The results showed that Bacillus strain JZB30-1 exhibited an obvious inhibitory effect on Botrytis cinerea,as well as broad-spectrum antifungal and antibacterial activities against more than ten plant pathogens.The strain was identified as Bacillus amyloliquefaciens,which have features of synthesizing protease,cellulase and lipopeptide,secreting siderophore,indoleacetic acid and solubilizing phosphate.The 50-fold sterile fermention filtrate of JZB30-1 displayed an obvious biocontrol effect on tomato fruit grey mould,with a 95.0％reduction in disease incidence compared to the control.In addition,the plant height and fresh weight increased by 25.7％and 28.4％,respectively,after root-irrigation with a 10-fold dilution of the fermentation.The study provides a theoretical foundation for considering B.amyloliquefaciens strain JZB30-1 as a potential biocontrol strain against tomato grey mould.
tomato grey mouldBacillus amyloliquefaciensantagonistic activityfunctional genesbiological control
万方数据
维普
