Analysis of active components in three host volatiles eliciting olfactory responses in Scotogramma trifolii
To investigate the active components of plant volatiles eliciting olfactory electrophysiological responses in Scotogramma trifolii,volatile compounds from Chenopodium album,Beta vulgaris,and Gossypium hirsutum.were collected using the dynamic headspace adsorption method.The active components in the host volatiles were qualitatively identified using gas chromatography electroantennography(GC-EAD)and gas chromatography-mass spectrometry(GC-MS).The results revealed that there are six active components in the volatile emissions of C.album,and five active components each in sugar beet and cotton volatiles.Among these,five components were common across all three plant volatiles,namely trans-3-hexenyl acetate,1,2-diethylbenzene,1,4-diethylbenzene,nonanal,and diethylene glycol monobutyl ether.Additionally,isophorone was exclusively found in the volatiles of C.album.The antennal electrophysiological responses(EAG)of S.trifolii reveal that the six active components can all cause EAG responses in both male and female adults,and increasing with increasing concentration,and the strongest EAG responses were observed to nonanal and isophorone,followed by trans-3-hexenyl acetate and 1,2-diethylbenzene.Furthermore,under the same concentration conditions,the EAG response of male and female adults to the same compound displayed no gender specificity.This study provides a basis for exploring the host localization mechanism of S.trifolii and for developing behavioral regulators.
万方数据
维普
