Trace determination of neonicotinoid insecticides imidacloprid,thiacloprid and acetamiprid and metabolites in rice and non-target aquatic organisms and its application
To investigate the residue properties and safety of neonicotinoid insecticides in rice and non-target aquatic organisms,a trace detection and analytical method for three neonicotinoid insecticides(imidacloprid,thiacloprid and acetamiprid)and their seven metabolites in rice,non-target aquatic organisms(Danio rerio and Daphnia magna)and water bodies was established.The samples were pretreated by QuEChERS method and detected by high performance liquid chromatography-tandem mass spectrometry.The target pesticides and their metabolites were quantified by external standard method.The results indicated that the solvent standard calibration curves and matrix standard calibration curves of the 10 target compounds showed good linearity in the range of 1-1 000 μg/kg with the determination coefficients of 0.991-0.999.The average recoveries of the 10 compounds in the six matrices ranged from 71.16％to 118.57％,and the relative standard deviations were in the range of 0.54％to 18.04％.The limits of quantification of the 10 target compounds in rice,D.rerio,nutrient solution and water were 10 μg/kg(50 μg/kg for D.magna),which satisfied the requirements of the residue analysis method.The established method was used to investigate the uptake,accumulation and metabolic behavior of three neonicotinoid insecticides in rice and non-target aquatic organisms(D.rerio and D.magna).The results showed that the transport factors of these three pesticides in rice were all greater than 7,which were easy to transport to above-ground parts,and the bioconcentration factors in non-target aquatic organisms were all less than 2 mL/g,indicating these three pesticides were low-bioconcentration pesticides.
万方数据
维普
