Identification and expression analysis of Vg genes in Ophraella communa
Vitellogenin(Vg)as a female multifunctional reproductive protein,which plays an important role in vitellogenesis.This study aimed to clarify the potential role of the Vg gene in the reproductive process of Ophraella communa.Three Vg genes were cloned by RT-PCR,and the open reading frames of OcVg 1,OcVg2,and OcVg3 were 5 310,5 322,and 5 298 bp,respectively,encoding 1 768,1 772,and 1 764 amino acids.OcVg proteins have the conserved domain of the LLTP superfamily,including LPD_N,DUF1943,and VWD domains.Polyserine domains located at the N-terminal,with the conserved GL/ICG,R/KXXR/K,DGXR motifs,and C-terminal cysteine residues.The OcVgs clustered with those of Coleoptera insects by constructing a phylogenetic tree.Spatio-temporal expression analysis showed that the expression dynamics of OcVg genes in different tissues and developmental stages were similar,with high specificity in fat bodies(P＜0.05).The expression level was the highest at the sexual maturity stage after adult emergence,and the expression level at the larval stage could be ignored(P＜0.05).The results of this study lay the foundation for a deeper understanding of the molecular mechanism of the reproductive function of O.communa.
万方数据
维普
