Rapid sex determination of pupae and adults of Serrodes campana(Lepidoptera:Noctuidae)
In this paper,by observing and comparing the external morphological differences between male and female individuals of the pupae and adults of Serrodes campana Guenée,characteristics and methods for distinguishing between the sexes of pupae and adults were summarized.The results showed that the 8th abdominal segment at the end of the abdomen of the female pupa has a longitudinal crack formed by the connection of the genital hole and the oviposition hole,forming a"herringbone"shape,and the two sides of the crack are flat without protrusions.The 8th abdominal segment of the male pupa has no slit,the 9th abdominal segment has a longitudinal crack near the center of the 10th abdominal segment,and there is a semicircular tumor-like protrusion on each side of the crack.Female and male adult morphological differences are large,the female adult sub middle fold has a fan-shaped medial black spot,and the forewing base line to the outer line is the same color as the pronotum,and the abdomen of female moth is more rounded than that of the male moth,and the abdomen is cone-shaped at the end of the abdomen.The black spot of male at the sub middle fold in front wings is nearly triangular,light yellow between the baseline and the outer line,the color is lighter than the pronotum,the abdomen is narrow and long,and the end of the abdomen is cylindrical.This method is simple and effective,and greatly improves the efficiency of distinguishing the sex of pupae and adults in the field,which is of great significance for the study of S.campana reproductive biology and subsequent extraction of sex pheromones.