Expression pattern of grape VvERF655 gene in response to Lasiodiplodia theobromae and interaction of VvERF655 and VvWRKY75
Botryosphaeria dieback,caused by Lasiodiplodia theobromae,is one of the main grapevine trunk diseases,significantly impacting grape production.Our previous study found that VvWRKY75 plays a negative regulatory role in the occurrence of Botryosphaeria dieback,and the co-expression network analysis revealed its co-expression with the gene VvERF655.To determine whether VvERF655 is involved in the regulation of Botryosphaeria dieback by grape,this study cloned VvERF655 gene and analyzed physical and chemical properties of VvERF655 protein by bioinformatics,alongside analyzing VvERF655 expression pattern via RT-qPCR.The results showed that VvERF655 responded to L.theobromae infection and was significantly induced by abscisic acid and salicylic acid.The interaction between VvWRKY75 and VvERF655 was further verified by yeast two-hybrid analysis.These results indicate that VvERF655 may be involved in the regulation of L.theobromae and hormonal responses in host grapes.This study lays a foundation for further elucidating the biological function of the VvERF655 gene in grapes.
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