Multiple resistance and target-based resistant mechanisms of Amaranthus retroflexus to herbicides in soybean fields
In Heilongjiang province,Amaranthus retroflexus has developed resistance to ALS(acetolactate synthase)and PPO(protoporphyrinogen oxidase)inhibitor herbicides in soybean fields.To understand the resistance mechanism of A.retroflexus to imazethapyr and fomesafen,a whole plant bioassay was conducted to determine the susceptibility of resistant and susceptible populations.The ALS and PPO genes(plastid PPX1 and mitochondrial PPX2)from the populations were cloned,and sequence alignment was conducted to find mutations.In addition,the mutated gene was transformed into Arabidopsis thaliana to verify its function.The results showed that the resistance factor for A.retroflexus resistant population to imazethapyr and fomesafen was 55.29 fold and 17.51 fold,respectively.The resistant plants contained the mutations Ala205Val or Ser653Asn in the ALS,and Arg128Gly in the PPX2.The A.thaliana containing mutated PPX2 gene showed high resistance to fomesafen(10.3 fold).In summary,mutations in ALS and PPO genes associate with multiple resistance of A.retroflexus to imazethapyr and fomesafen in soybean fields in northeast China.
NETL
NSTL
万方数据
