大豆田反枝苋对除草剂多抗性及靶标抗性机理研究
Multiple resistance and target-based resistant mechanisms of Amaranthus retroflexus to herbicides in soybean fields
杜龙 1李宁 2尚素琴 3李丹 3李晓 1曲明静1
作者信息
- 1. 山东省花生研究所,国家花生工程技术研究中心,青岛 266100
- 2. 黄冈市农业科学院,黄冈 438000
- 3. 甘肃农业大学植物保护学院,兰州 730070
- 折叠
摘要
黑龙江省大豆田反枝苋Amaranthus retroflexus对ALS、PPO抑制剂类除草剂产生了严重抗性,为明确化学防除失败的反枝苋对咪唑乙烟酸、氟磺胺草醚的抗性机理,采用整株生物测定法测定了反枝苋抗性及敏感种群对除草剂的敏感性,克隆了抗性和敏感种群的ALS、PPO基因(质体型PPX1及线粒体型PPX2),查找突变位点,将突变基因转入拟南芥Arabidopsis thaliana验证其功能,明确抗性机理.结果显示:反枝苋抗性种群对咪唑乙烟酸、氟磺胺草醚抗性倍数分别为55.29倍和17.51倍,抗性植株ALS存在Ala205Val或Ser653Asn突变,PPX2存在Arg128Gly突变.将抗性种群的PPX2转入拟南芥,可导致拟南芥对氟磺胺草醚产生高水平抗性(10.3倍).综上,东北大豆田反枝苋对咪唑乙烟酸、氟磺胺草醚产生了高水平多抗性,ALS、PPO基因突变是导致此类抗性的主要原因之一.
Abstract
In Heilongjiang province,Amaranthus retroflexus has developed resistance to ALS(acetolactate synthase)and PPO(protoporphyrinogen oxidase)inhibitor herbicides in soybean fields.To understand the resistance mechanism of A.retroflexus to imazethapyr and fomesafen,a whole plant bioassay was conducted to determine the susceptibility of resistant and susceptible populations.The ALS and PPO genes(plastid PPX1 and mitochondrial PPX2)from the populations were cloned,and sequence alignment was conducted to find mutations.In addition,the mutated gene was transformed into Arabidopsis thaliana to verify its function.The results showed that the resistance factor for A.retroflexus resistant population to imazethapyr and fomesafen was 55.29 fold and 17.51 fold,respectively.The resistant plants contained the mutations Ala205Val or Ser653Asn in the ALS,and Arg128Gly in the PPX2.The A.thaliana containing mutated PPX2 gene showed high resistance to fomesafen(10.3 fold).In summary,mutations in ALS and PPO genes associate with multiple resistance of A.retroflexus to imazethapyr and fomesafen in soybean fields in northeast China.
关键词
反枝苋/氟磺胺草醚/咪唑乙烟酸/多抗性机理Key words
Amaranthus retroflexus/fomesafen/imazethapyr/multiple resistance mechanism引用本文复制引用
基金项目
山东省自然科学基金(ZR2020QC135)
国家自然科学基金(32001940)
国家花生产业技术体系建设专项(CARS-13)
出版年
2024
NETL
NSTL
万方数据