Identification and complete genome sequence analysis of soybean mosaic virus from soybean in Hebei,China
In this study,six isolates of soybean mosaic virus(labelled SMV-Gm1 to SMV-Gm6)were detected from soybean in Lulong,Hebei province using small RNA deep sequencing technology.Based on the sequencing results and a reference genome sequence,specific primers were designed to clone the genome sequences of these SMV isolates.Following assembly,six full-length SMV genome sequences were obtained,with sizes of 9 588 nt for SMV-Gm1,SMV-Gm3,and SMV-Gm5,and 9 584 nt for SMV-Gm2,SMV-Gm4,and SMV-Gm6.The open reading frame is located between 132nd to 9 332nd nucleotides in each genome,encoding a polyprotein with a molecular weight of approximately 350 kD.BLAST alignment and phylogenetic analysis showed that SMV-Gm1.SMV-Gm3,and SMV-Gm5 showed the highest sequence similarity(98.06％to 98.07％)and relatively close genetic distance with the SMV isolate from Jiangsu province(accession number:MH919386),clustering with SMV isolates from Jiangsu,Zhejiang and Shanxi provinces.Similarly,the nucleotide sequences of SMV-Gm2,SMV-Gm4,and SMV-Gm6 exhibited the highest similarity(98.48％to 98.51％)with the SMV isolate from South Korea(accession number:FJ640954),indicating a close genetic relationship.
soybean mosaic virus(SMV)small RN A sequencinggenome sequence
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NSTL
万方数据
