两种林木植原体病害的分子鉴定
Molecular identification of two woody plant phytoplasma diseases
辛霁航 1崔亚琴 2刘红霞 1游崇娟1
作者信息
- 1. 北京林业大学林木有害生物防治北京市重点实验室,北京 100083
- 2. 山西省林业和草原科学研究院,太原 030012
- 折叠
摘要
近年来,林木植原体病害发生日趋严重,对我国林业经济和生态造成了很大损失.2021年-2022年,在山西沙棘主产区的沙棘上和北京冬奥园区的油松上分别出现了典型的植原体病害症状;沙棘叶片皱缩,呈小叶状;油松过度分枝生长且出现球状结构等.本研究通过荧光显微观察,16S rRNA和tuf基因序列分析,并结合虚拟限制性片段长度多态性分析证实了这两种病害均由植原体引起.基于16S rRNA的系统进化分析显示:引起沙棘叶片皱缩的植原体与泡桐丛枝植原体(Paulownia witches'-broom phytoplasma,OP107515.1)的相似性最高(99.92％),引起油松丛枝的植原体与狗尾草丛枝植原体株系(Setaria viridis witches'-broom phytoplasma,FJ263625.1)的相似性最高(99.52％);基于tuf基因的系统发育树显示,二者同属于16Sr Ⅰ组D亚组(16Sr Ⅰ-D).本研究首次明确了沙棘叶片皱缩病和油松丛枝病相关植原体的分类地位,为这两种植原体病害的准确诊断、快速检测及其防治提供了基础资料.
Abstract
In recent years,woody plant phytoplasma diseases have become increasingly serious,resulting in huge economic and ecological losses in China.During 2021-2022,serious phytoplasma diseases were found in the main Hippophae rhamnoides production areas in Shanxi province and Pinus tabuliformis in Beijing Winter Olympic Park.Typical symptoms associated with phytoplasma,including crumpled and leaflet-like leaves on H.rhamnoides and abnormal shoot proliferation with spherical structure on P.tabuliformis were observed.The pathogens causing these two diseases were identified as phytoplasma based on a combination of fluorescence microscopic morphological observation,sequences analysis of 16S rRNA and tuf gene,and computer-simulated restriction fragment length polymorphism analysis.The 16S rRNA gene analysis revealed that phytoplasma causing leaf wrinkling in H.rhamnoides and witches'-broom in P.tabuliformis showed 99.92％similarity with Paulownia witches'-broom phytoplasma(OP107515.1)and 99.52％similarity with Setaria viridis witches'-broom phytoplasma(FJ263625.1),respectively.The phylogenetic tree based on the tuf gene showed that both pathogens belonged to the 16Sr Ⅰ group D subgroup(16Sr Ⅰ-D).Our study demonstrated the taxonomic status of these two pathogens for the first time and provides the basic data for the accurate detection and control of these two diseases in the future.
关键词
植原体/沙棘/油松/16SrRNA/tuf基因/RFLP分析Key words
phytoplasma/Hippophae rhamnoides/Pinus tabuliformis/16S rRNA/tuf gene/RFLP analysis引用本文复制引用
基金项目
国家重点研发计划(2021YFC2600400)
山西省林业重点研发计划专项(LYZDYF2023-22)
出版年
2024
NETL
NSTL
万方数据