Molecular identification of two woody plant phytoplasma diseases
In recent years,woody plant phytoplasma diseases have become increasingly serious,resulting in huge economic and ecological losses in China.During 2021-2022,serious phytoplasma diseases were found in the main Hippophae rhamnoides production areas in Shanxi province and Pinus tabuliformis in Beijing Winter Olympic Park.Typical symptoms associated with phytoplasma,including crumpled and leaflet-like leaves on H.rhamnoides and abnormal shoot proliferation with spherical structure on P.tabuliformis were observed.The pathogens causing these two diseases were identified as phytoplasma based on a combination of fluorescence microscopic morphological observation,sequences analysis of 16S rRNA and tuf gene,and computer-simulated restriction fragment length polymorphism analysis.The 16S rRNA gene analysis revealed that phytoplasma causing leaf wrinkling in H.rhamnoides and witches'-broom in P.tabuliformis showed 99.92％similarity with Paulownia witches'-broom phytoplasma(OP107515.1)and 99.52％similarity with Setaria viridis witches'-broom phytoplasma(FJ263625.1),respectively.The phylogenetic tree based on the tuf gene showed that both pathogens belonged to the 16Sr Ⅰ group D subgroup(16Sr Ⅰ-D).Our study demonstrated the taxonomic status of these two pathogens for the first time and provides the basic data for the accurate detection and control of these two diseases in the future.
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