Effect of recommended field dose of chlorantraniliprole on the growth,development and endosymbionts of Binodoxys communis
Chlorantraniliprole(CAP)is a new type of formamide benzamide insecticide widely used in agricultural production.The cotton aphid,Aphis gossypii Glover,is a common pest with global distribution.Binodoxys communis(Gahan)is a dominant parasitic wasp of A.gossypii.This study aimed to assess the effects of CAP at the recommended field dose(66.7 mg/L)on the survival rate,survival time,and endosymbionts of B.communis.The results showed that compared with the control,CAP exposure had no significant effect on the survival rate of tested generation and its offspring of B.communis,but leading to the shortened survival time of F0 generation(CK:4.6 d,CAP:2.9 d)and F1 generation(CK:4.6 d;CAP:3.4 d)when exposure for one hour.High-throughput sequencing of 16S rDNA showed that CAP-exposure for one hour or three days did not affect the endosymbionts community structure in B.communis,but the relative abundance of endosymbionts in B.communis changes after CAP-exposure for three days.At the phylum level,the abundance of Proteobacteria significantly increased(CK:35.5％;CAP:99.68％),while at the genus level,Serratia abundance significantly increased(CK:7.84％;CAP:36.82％),and the abandance of Rhodococcus decreased(CK:64.43％,CAP:0).In conclusion,the recommended field dose of CAP adversely affected the survival of both F0 and F1 generations,accompanied by alterations in the relative abundance of dominant genera in their bodies.These results hold important significance in evaluating the safety of CAP to parasitic natural enemies.The management of chlorantraniliprole should be strengthened to prevent its abuse in field environments,thereby averting toxicity problems and safeguarding environmental safety.
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