Sublethal effects of tolfenpyrad and its impact on detoxifying enzymes to Spodoptera exigua
To clarify the sublethal effects of tolfenpyrad to Spodoptera exigua and biochemical resistance mechanism of S.exigua to tolfenpyrad,the indoor toxicity of 10 different pesticides against SS.exigua were measured and the impacts of tolfenpyrad at LC10,LC30,and LC50 concentrations on the growth,reproduction,detoxifying enzyme activity were assessed,and biochemical resistance mechanism of the S.exigua to tolfenpyrad was explored.The results indicated that the adult lifespan,emergence rate,and egg hatching rate of S.exigua in high concentration treatment group(LC50)were significantly lower than those in the control group.Low concentration of tolfenpyrad(LC10)induced an increase in the activity of glutathione S-transferase(GST)and carboxylesterase(CarE)in S.exigua,while significantly inhibiting the activity of multifunctional oxidase(MFO).Synergism tests showed that piperonyl butoxide(PBO)and triphenyl phosphate(TPP)exhibited noticeable synergistic effects on tolfenpyrad.The enzyme activities of CarE,MFO,and GST in the resistant population were significantly higher than those in the susceptible population.In summary,low concentrations of tolfenpyrad exert a significant inhibitory effect on the growth and development of S.exigua,with MFO,CarE and GST playing crucial roles in its resistance to tolfenpyrad.
NETL
NSTL
万方数据
