Cloning of the cytochrome P450 gene CYP6JM1 and its function in thiamethoxam resistance in the whitefly,Bemisia tabaci
Whitefly,Bemisia tabaci,are among the most damaging agricultural pests in the world,and chemical pesticides have been widely applied to control them.Thiamethoxam,a neonicotinoid insecticide,has been widely used to control B.tabaci in the field,but the issue of whitefly resistance has emerged alongside the abuse of pesticides.This study utilized quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)to compare the expression levels of the cytochrome P450 gene CYP6JM1 between thiamethoxam-resistant and susceptible populations of B.tabaci.The results indicated a significant increase in the expression level of the CYP6JM1 gene in the thiamethoxam resistant population.Following gene cloning and sequence analysis,it was confirmed that the CYP6JM1 gene exhibited typical characteristics of insect P450 genes.Moreover,RT-qPCR showed that this gene was over-expressed in the 1st-to 2nd-instar nymph and abdomen of B.tabaci.RNA interference(RNAi)experiments showed that reducing the expression of CYP6JM1 gene significantly enhanced sensitivity to thiamethoxam,suggesting that the CYP6JM1 gene may be involved in the formation of resistance to thiamethoxam.
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