西瓜叶斑病病原菌的鉴定及室内药剂筛选
Identification of the pathogen causing leaf spot on watermelon and fungicide screening in vitro
曾蓉 1徐丽慧 1陈灏岚 2成玮 3高萍 1高士刚 1宋志伟 1戴富明1
作者信息
- 1. 上海市农业科学院生态环境保护研究所,上海市设施园艺重点实验室,上海 201403
- 2. 上海市浦东新区农业技术推广服务中心,上海 201323
- 3. 上海市农业技术推广服务中心,上海 201799
- 折叠
摘要
2022年在上海市浦东新区西瓜上发生一种深褐色、不规则至近圆形斑的叶部病害.采用组织分离法对病害样本进行分离,分离物SHCCC01经柯赫氏法则验证具有较强致病性.根据分离物的菌落特征、分生孢子形态、生物学特征以及基于核糖体内部转录间隔区(internal transcribed spacer,ITS)、组蛋白基因(histone,His)、真核翻译延伸因子1α(translation elongation factor 1α,TEF1-α)和肌动蛋白(actin,Act)基因联合系统发育分析等,将病原菌鉴定为瓜类尾孢Cercospora citrullina.10种杀菌剂对该病原菌的毒力测定试验结果表明:氟啶胺、己唑醇、氟环唑、戊唑醇等4种药剂对病原菌的抑菌活性较高,其EC50分别为0.375 2、0.575 4、1.472 3、1.890 2 μg/mL.这些结果为西瓜尾孢叶斑病的防控和杀菌剂登记提供了重要依据.
Abstract
A kind of watermelon leaf spot disease was found in Pudong district,Shanghai.The isolates were obtained from diseased samples by tissue isolation method.Pathogenicity of the isolate SHCCC01 was verified by Koch's postulates.The pathogen was identified by morphology,biological characteristics,and multilocus phylogenetic analysis based on the internal transcribed spacer(ITS),histone(His),translation elongation factor 1α(TEF1-α)and actin(Act).The results showed that the pathogen of the watermelon leaf spot disease was Cercospora citrullina.Among the ten tested fungicides,fluazinam,hexaconazole,epoxiconazole and tebuconazole showed strong inhibition to hyphal growth of C.citrullina isolate SHCCC01 with the EC50 value of 0.375 2,0.575 4,1.472 3 and 1.890 2 μg/mL,respectively.These results provide important theoretical basis for control of watermelon Cercospora leaf spot disease and registration of fungicides.
关键词
西瓜/瓜类尾孢/多基因序列联合分析/毒力测定Key words
watermelon/Cercospora citrullina/multilocus phylogenetic analysis/toxicity引用本文复制引用
基金项目
上海市西瓜甜瓜产业技术体系建设(沪农科产字2022第1号)
上海市农业科学院生态所揭榜挂帅(生科-JB2023-2)
出版年
2024
国家科技期刊平台
NSTL
万方数据