Wettable powder preparation of Botrytis strain HY-021 and its herbicidal effect
In order to clarify the herbicidal activity of the Botrytis strain HY-021,a wettable powder was prepared,and its herbicidal activity was measured.The single-factor method was used to screen the optimal solid fermentation substrate,suitable carrier,and auxiliary agent for the strain HY-021.A wettable powder was prepared and its herbicidal activity was evaluated by pot inoculation.The results showed that the most suitable solid substrate for the strain HY-021 to grow was corn flour,and the optimal formula combination was wheat straw bran 14.5 g,wheat bran 19.4 g,rapeseed cake 1.5 g,and maize flour 14.6 g.Kaolin,light calcium carbonate,carboxymethylcellulose sodium,soluble starch and sodium dodecyl sulfate were comprehensively selected as the carrier,stabilizer,dispersant,protector,and wetting agent of the strain HY-021.The wettable powder of the strain HY-021 exhibited good herbicidal activity against three kinds of weeds,namely Chenopodium album,Elsholtzia densa,and Galium spurium,at the seedling stage in pot tests.Among them,the HY-021 showed strong pathogenicity to C.album and G.spurium,with incidence rate of 85.83％and 89.45％,and fresh weight control effect of 81.82％and 87.16％,respectively.HY-021 wettable powder can be used as a biocontrol agent to effectively control weeds such as C.album and G.spurium.
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