树生黄单胞菌李致病变种两种PCR检测方法的建立
Two specific PCR assays for detection of Xanthomonas arboricola pv.pruni
杨雯迪 1马玉超 1王金鹏 1沈建国 2任争光 1杨海清 3李为民1
作者信息
- 1. 北京农学院植物保护系,农业农村部华北都市农业重点实验室,北京 102206
- 2. 福建省检验检疫技术研究重点实验室,福州海关技术中心,福州 350001
- 3. 平谷区果品产业服务中心,北京 101200
- 折叠
摘要
树生黄单胞菌李致病变种Xanthomonas arboricola pv.pruni(Xap)是桃、李生产中最具毁灭性的病原细菌之一.为了快速准确地检测Xap,本研究以其编码基因L1B16_06350的5'端序列(611 bp)为检测靶标,开发了常规PCR和TaqMan实时PCR检测方法.这两种方法与树生黄单胞菌胡桃致病变种X.arboricola pv.juglandis等8个不同属的11种细菌均没有交叉反应,其中常规PCR检测极限为10-2 ng,TaqMan探针法为1(-3 ng,表现高度的灵敏度和特异性.利用田间样品进行测试,结果证实两种检测方法均可应用于Xap的常规检测.
Abstract
Xanthomonas arboricola pv.pruni(Xap)is one of the most devastating bacterial pathogen to peach and plum.To rapidly and accurately detect Xap,we developed a conventional PCR and a TaqMan real-time PCR assay that targets the 5'end(611 bp)of L1B16_06350,a Xap encoding gene.Both methods had no cross-reaction with 11 tested bacterial species from eight different genera,including X.arboricola pv.juglandis etc.,with a limit of detection of 10-2ng for the conventional PCR and 10-3 ng for the TaqMan method,respectively,showing high sensitivity and specificity.Field sample test proved the reliability of the two methods in routine diagnosis of Xap.
关键词
树生黄单胞菌李致病变种/常规PCR/TaqMan探针法/特异性/灵敏度Key words
Xanthomonas arboricola pv.pruni/conventional PCR/TaqMan real-time PCR/specificity/sensitivity引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据