Two specific PCR assays for detection of Xanthomonas arboricola pv.pruni
Xanthomonas arboricola pv.pruni(Xap)is one of the most devastating bacterial pathogen to peach and plum.To rapidly and accurately detect Xap,we developed a conventional PCR and a TaqMan real-time PCR assay that targets the 5'end(611 bp)of L1B16_06350,a Xap encoding gene.Both methods had no cross-reaction with 11 tested bacterial species from eight different genera,including X.arboricola pv.juglandis etc.,with a limit of detection of 10-2ng for the conventional PCR and 10-3 ng for the TaqMan method,respectively,showing high sensitivity and specificity.Field sample test proved the reliability of the two methods in routine diagnosis of Xap.
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