Screening,identification and efficacy of biocontrol fungi against southern root-knot nematode
The root-knot nematode(Meloidogyne spp.)is one of the most common and harmful plant parasitic nematodes,causing significant losses to agricultural production.The fungus strain Snef2766 was screened in this study,which showed high nematocidal activity against Meloidogyne incognita second-stage juveniles(J2)and could effectively inhibit egg hatching.Based upon morphological characteristics and ITS sequence analysis,strain Snef2766 was identified as Aspergillus insuetus.Pot experiments demonstrated that root irrigation with fermentation filtrates of the strain Snef2766 significantly reduced root knots of tomato plant and egg masses,with the reduction rates of 64.22％and 58.70％,respectively,and promoted the growth of tomato plants.Greenhouse experiment showed that strain Snef2766 could effectively control root-knot nematode disease by seed-coating and root irrigation treatment,with the control efficacy of 60.69％and 63.55％,respectively,and promote the growth of tomato.Therefore,A.insuetus Snef2766 had a significant effect on promoting growth and controlling southern root-knot nematode disease.This is the first report of A.insuetus as a biocontrol agent against M.incognita,which provide a new microbial resource for the biological control of root-knot nematode disease.
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